Journal: Pathogens and disease

Article Title: Hyperglycemia suppresses the regulatory effect of hypoxia-inducible factor-1α in pulmonary Aspergillus fumigatus infection.

doi: 10.1093/femspd/ftaa038

Figure Lengend Snippet: Figure 2. Hyperglycemia suppressed HIF-1α and overactivated NLRP3/IL-1β signal pathway in A. fumigatus-activated macrophages in hypoxia condition. 5.5 or 30 mM glucose concentration was used to culture the A. fumigatus-activated macrophages for 0, 12 and 24 h. (A) HIF-1α, NLRP3 and β-actin protein levels were detected using western blotting in the A. fumigatus-activated macrophages. Representative images of HIF-1α, NLRP3 and β-actin western blots. (B) Quantification of HIF-1α and NLRP3 protein expression normalized to β-actin. (C) Culture supernatants were examined for IL-1β secretion using ELISA. Data are shown as mean ± SD (n = 3). ∗, P < 0.05, ∗∗, P < 0.01. #, P < 0.05, ##, P < 0.01: compared to 0 h for the same group. Analyzed using paired Student’s t-test and RM one-way ANOVA.

Article Snippet: Analysis of A. fumigatus challenged mice The concentration of Aspergillus Antigen in serum samples was measured by enzyme linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Bio-Rad, MamesLa-Coquette, France).

Techniques: Concentration Assay, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Pathogens and disease

Article Title: Hyperglycemia suppresses the regulatory effect of hypoxia-inducible factor-1α in pulmonary Aspergillus fumigatus infection.

doi: 10.1093/femspd/ftaa038

Figure Lengend Snippet: Figure 4. DMOG and Alum regulated HIF-1α activation and NLRP3/IL-1β signal pathway in A. fumigatus-activated macrophages in hyperglycemic hypoxia state. The macrophages were pre-incubated with 200 μM DMOG + 50 μg/ml Alum, 200 μM DMOG or control-DMSO for 2 h before stimulation with microbial stressors under 30 mM glucose concentration, and then were cultured for 12, 24 h. (A) HIF-1α, NLRP3 and β-actin protein levels were detected using western blotting in the A. fumigatus- activated macrophages. Representative images of HIF-1α, NLRP3 and β-actin western blots. (B) Quantification of HIF-1α and NLRP3 protein expression normalized to β-actin. (C) Culture supernatants were examined for IL-1β secretion using ELISA. Data are shown as mean ± SD (n = 3). ∗, P < 0.05, ∗∗, P < 0.01. Analyzed using two-way ANOVA followed by Bonferroni post hoc test.

Article Snippet: Analysis of A. fumigatus challenged mice The concentration of Aspergillus Antigen in serum samples was measured by enzyme linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Bio-Rad, MamesLa-Coquette, France).

Techniques: Activation Assay, Incubation, Control, Concentration Assay, Cell Culture, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay